Amit Mishra (Postdoctoral Fellow in Max Planck Institute, Martinsried (Cell Biochemistry)
PhD. in Neuroscience
(NATIONAL BRAIN RESEARCH CENTRE, INDIA)
Cellular and Molecular Neuroscience Lab, Manesar
Interest:- My principal examine awareness is neurodegenerative diseases and neurodevelopment disorders. I am enthralled to find the role of Ubiquitin ligase in non-native protein degradation. Also I am truly attracted toward cell cycle and cancer research area.
Summary:- Highly comfortable touch with protein science. Strong background in protein expression and degradation. Personal interest in Cellular and Molecular neuroscience analysis. I am always feeling comfort to perform work in the scientific leadership of a cellular and molecular bioscience oriented screening center. Freedom to continue work at the cutting edge of this field is a must.
Professional experience and education:-
Senior Research Fellow (Ph.D. in Neuroscience)2003- Defence remain
Course work:- IST Division
Current work:- Conducting an in-depth analysis of every aspect of ubiquitin ligase (UBE3A) gene for its contribution to possible substrate degradation. Seeking UBE3A role in neurodegenrative diseases.
Thesis title:- “Understanding the molecular function of E6-AP-a putative E3 ubiquitin ligase mutated in Angelman mental retardation syndrome”
Developed solid background in :-
Animal handling, Cell/Tissue Culture, Cloning, Electrophoresis,Electron microscopy,Gene transfer/Targeting, High-Throughput Screening, Image Analysis, Immunotechnology/,InsInsitu Hybridization, Microscopy, Model Organisms, Molecular Engineering Mutagenesis, Nucleic Acid Hybridization, Nucleic Acid Isolation, PCR, Protein Expression, Protein-Protein Interaction, Protein Purification, Protein Array, RNAi/Antisense, and Sequence analysis.
Non-Biological Skills- Web page development, Mat lab, Soundknowledge of several software’s and Hardware’s.
M.S. in Biotechnology: - GGPA-8.5/10(with Honor) Marks inPercentage- 79 (2001-2003)
2003-Score 99.05, GATE (Graduate Aptitude Test in Engineering )Exam by IIT Madras,All India Rank- 31
2001- Cleared All India M.S in Biotechnology test by DBT New Delhi
2001- All India Rank 04 in B.Sin Pharmaceutical
1995-2001 National Talent Search fellowship,India (Merit Scholars)
Grants/fellowships awarded:-
2007- Riken Brain Science Institute- Japan Summer Research fellowship
2006-2007– Best Presetation Award (IST Prize)-S. S. Parmar Foundation Prize, USA
2003-2008- Council of Scientific and Industrial Research, India fellowship
2001-2003- Department of Biotechnology, India MS fellowship
Publications: -
1. Amit Mishra, Priyanka Dikshit, Sudarshana Purkayastha, Jaiprakash Sharma, Nobuyuki Nukina, and Nihar Ranjan Jana E6-AP promotes misfolded polyglutamine proteins for proteasomal degradation and suppresses polyglutamine protein aggregation and toxicity "Journal of Biological Chemistry". 2008 Mar 21;283(12):7648-56. Epub 2008 Jan 17.
2. A.Mishra and N.R.Jana
Regulation of turnover of tumor suppressor p53 and cell growth by E6-AP, a ubiquitin protein ligase mutated in Angelman mental retardation syndrome. "Cellular and Molecular life sciences"2007 (Accepeted)
3. A.Goswami, P.Dikshit, A.Mishra, N.Nukina, N.R.Jana Expression of expanded polyglutamine proteins suppresses the activation of transcription factor NFkappaB. "Journal of Biological Chemistry". 2006 Dec 1;281(48):37017-24.
4. P.Dikshit, Chatterjee M, A.Goswami, A.Mishra, N.R.Jana Aspirin induces apoptosis through the inhibition of proteasome function. "Journal of Biological Chemistry". 2006 Sep 29;281(39):29228-35. Epub 2006 Jul 31.
5. P.Dikshit, A.Goswami, A.Mishra, N.Nukina and N.R.Jana Curcumin enhances the polyglutamine-expanded truncated N-terminal huntingtin-induced cell death by promoting proteasomal malfunction. "Biochemical and Biophysical Research Communication". 2006 Apr 21;342(4):1323-8. Epub 2006 Feb 28.
6. A.Goswami, P.Dikshit, A.Mishra, S.Mulherkar, N.Nukina and N.R. Jana Oxidative stress promotes mutant huntingtin aggregation and mutant huntingtin-dependent cell death by mimicking proteasomal malfunction. "Biochemical and Biophysical Research Communication". 2006 Mar 31;342(1):184-90. Epub 2006 Feb 3.
7.P.Dikshit, A.Goswami, A.Mishra, Chatterjee M, Jana NR. Curcumin induces stress response, neurite outgrowth and prevent NF-KB activation by inhibiting the proteasome function. "Neurotoxicity Research".
2006 Jan;9(1):29-37.
International Presentations: -
A.Mishraand N.R Jana.
Role of E6-AP acting as a E3 ubiquitin ligase in molecular pathogenesis mechanism of neurodevelopmental disorder and neurodegenrative diseases. Max Planck Institute, Department Cellular Biochemistry, Martinsried, Germany. 2008
A.Mishra and N.R. Jana.
Angelman ubiquitin ligase promotes ubiquitin mediated degradation of tumor suppressor p53 in the neuronal cells. RIKEN Brain Science Institute, Japan, 2007
Goswami A, Dikshit P, Mishra A, Nukina N and Jana NR. Expression of expanded polyglutamine proteins suppresses the activation of transcription factor NF-kB. At Japanese Neuroscience Society meeting held in Kyoto (JAPAN)2006
National Presentations: -
A.Mishra and N.R. Jana.
Angelman ubiquitin ligase E6-AP promotes degradation of tumor suppressor p53 by Proteasome in the neuronal cells. Silver Jubilee of Indian Academy of Neuroscience 2006.
P. Dikshit, A. Goswami, A.Mishraand N. R. Jana. Curcumin induces apoptosis through the impairment of ubiquitin proteasome pathway. Annual meeting of the Indian Association for Cancer Research, Noida, 2005.
